ER00305
Vacuum Filter 150 ml, With 50 mm 0.22 µm Nylon Filter
150 ml, 50 mm, 0.22 µm, Nylon
Lead time 3-4 weeks, ships from the Czech Republic (EU)
€10.73 per piece
Vacuum filter units
Vacuum filter units for 150, 250, 500 and 1000 ml, with 50, 75 or 91 mm membranes in PES, PVDF or nylon, 0.22 or 0.45 µm.
They are used to filter cell culture media, buffers and mobile phases under vacuum. Items not in stock have a lead time of 3 to 4 weeks.
- Products
- 24
Quick orderPaste SKUs and quantities from your spreadsheet or PO
Products
ER00306
Vacuum Filter 150 ml, With 50 mm 0.22 µm PES Filter
150 ml, 50 mm, 0.22 µm, PES
Lead time 3-4 weeks, ships from the Czech Republic (EU)
€10.73 per piece
ER00307
Vacuum Filter 150 ml, With 50 mm 0.22 µm PVDF Filter
150 ml, 50 mm, 0.22 µm, PVDF
Lead time 3-4 weeks, ships from the Czech Republic (EU)
€10.73 per piece
ER00308
Vacuum Filter 150 ml, With 50 mm 0.45 µm Nylon Filter
150 ml, 50 mm, 0.45 µm, Nylon
Lead time 3-4 weeks, ships from the Czech Republic (EU)
€10.73 per piece
ER00309
Vacuum Filter 150 ml, With 50 mm 0.45 µm PES Filter
150 ml, 50 mm, 0.45 µm, PES
Lead time 3-4 weeks, ships from the Czech Republic (EU)
€10.73 per piece
ER00310
Vacuum Filter 150 ml, With 50 mm 0.45 µm PVDF Filter
150 ml, 50 mm, 0.45 µm, PVDF
Lead time 3-4 weeks, ships from the Czech Republic (EU)
€10.73 per piece
ER00311
Vacuum Filter 250 ml, With 50 mm 0.22 µm Nylon Filter
250 ml, 50 mm, 0.22 µm, Nylon
Lead time 3-4 weeks, ships from the Czech Republic (EU)
€13.79 per piece
ER00312
Vacuum Filter 250 ml, With 50 mm 0.22 µm PES Filter
250 ml, 50 mm, 0.22 µm, PES
Lead time 3-4 weeks, ships from the Czech Republic (EU)
€13.79 per piece
ER00313
Vacuum Filter 250 ml, With 50 mm 0.22 µm PVDF Filter
250 ml, 50 mm, 0.22 µm, PVDF
Lead time 3-4 weeks, ships from the Czech Republic (EU)
€13.79 per piece
ER00314
Vacuum Filter 250 ml, With 50 mm 0.45 µm Nylon Filter
250 ml, 50 mm, 0.45 µm, Nylon
Lead time 3-4 weeks, ships from the Czech Republic (EU)
€13.79 per piece
ER00315
Vacuum Filter 250 ml, With 50 mm 0.45 µm PES Filter
250 ml, 50 mm, 0.45 µm, PES
Lead time 3-4 weeks, ships from the Czech Republic (EU)
€13.79 per piece
ER00316
Vacuum Filter 250 ml, With 50 mm 0.45 µm PVDF Filter
250 ml, 50 mm, 0.45 µm, PVDF
Lead time 3-4 weeks, ships from the Czech Republic (EU)
€13.79 per piece
ER00317
Vacuum Filter 500 ml, With 75 mm 0.22 µm Nylon Filter
500 ml, 75 mm, 0.22 µm, Nylon
Lead time 3-4 weeks, ships from the Czech Republic (EU)
€15.33 per piece
ER00318
Vacuum Filter 500 ml, With 75 mm 0.22 µm PES Filter
500 ml, 75 mm, 0.22 µm, PES
Lead time 3-4 weeks, ships from the Czech Republic (EU)
€15.33 per piece
ER00319
Vacuum Filter 500 ml, With 75 mm 0.22 µm PVDF Filter
500 ml, 75 mm, 0.22 µm, PVDF
Lead time 3-4 weeks, ships from the Czech Republic (EU)
€15.33 per piece
ER00320
Vacuum Filter 500 ml, With 75 mm 0.45 µm Nylon Filter
500 ml, 75 mm, 0.45 µm, Nylon
Lead time 3-4 weeks, ships from the Czech Republic (EU)
€15.33 per piece
ER00321
Vacuum Filter 500 ml, With 75 mm 0.45 µm PES Filter
500 ml, 75 mm, 0.45 µm, PES
Lead time 3-4 weeks, ships from the Czech Republic (EU)
€15.33 per piece
ER00322
Vacuum Filter 500 ml, With 75 mm 0.45 µm PVDF Filter
500 ml, 75 mm, 0.45 µm, PVDF
Lead time 3-4 weeks, ships from the Czech Republic (EU)
€15.33 per piece
ER00323
Vacuum Filter 1000 ml, With 91 mm 0.22 µm Nylon Filter
1000 ml, 91 mm, 0.22 µm, Nylon
Lead time 3-4 weeks, ships from the Czech Republic (EU)
€21.46 per piece
ER00324
Vacuum Filter 1000 ml, With 91 mm 0.22 µm PES Filter
1000 ml, 91 mm, 0.22 µm, PES
Lead time 3-4 weeks, ships from the Czech Republic (EU)
€21.46 per piece
ER00325
Vacuum Filter 1000 ml, With 91 mm 0.22 µm PVDF Filter
1000 ml, 91 mm, 0.22 µm, PVDF
Lead time 3-4 weeks, ships from the Czech Republic (EU)
€21.46 per piece
ER00326
Vacuum Filter 1000 ml, With 91 mm 0.45 µm Nylon Filter
1000 ml, 91 mm, 0.45 µm, Nylon
Lead time 3-4 weeks, ships from the Czech Republic (EU)
€21.46 per piece
ER00327
Vacuum Filter 1000 ml, With 91 mm 0.45 µm PES Filter
1000 ml, 91 mm, 0.45 µm, PES
Lead time 3-4 weeks, ships from the Czech Republic (EU)
€21.46 per piece
ER00328
Vacuum Filter 1000 ml, With 91 mm 0.45 µm PVDF Filter
1000 ml, 91 mm, 0.45 µm, PVDF
Lead time 3-4 weeks, ships from the Czech Republic (EU)
€21.46 per piece
No products match these filters.
A syringe filter removes particles from a liquid sample before it goes into an HPLC, GC or other instrument, protecting the column and the injector. Three choices decide which filter you need: the membrane, the pore size and the diameter.
Step 1: Membrane: match it to your solvent
| Membrane | Use it for | Avoid |
|---|---|---|
| Nylon | Most aqueous and organic HPLC samples; a good general-purpose choice | Protein samples (binds protein), strong acids |
| Hydrophilic PTFE | Mixed aqueous/organic HPLC samples, low extractables | - |
| Hydrophobic PTFE | Pure organic solvents, aggressive chemicals, gases and venting | Aqueous samples unless pre-wetted with methanol or IPA |
| PES | Aqueous and biological samples, cell culture media; low protein binding | Strong organic solvents |
| PVDF (hydrophilic) | Protein and biological samples, aqueous and mild organic solutions | Strong bases |
| MCE | Aqueous solutions and general clarification | Organic solvents |
Step 2: Pore size
0.45 µm is the standard for clarifying samples before HPLC. 0.22 µm (also written 0.2 µm) is used for UHPLC with sub-2 µm columns and for removing bacteria when the filter is sterile.
Step 3: Diameter: match it to the volume
| Diameter | Typical sample volume |
|---|---|
| 13 mm | Up to about 10 ml; least sample lost in the housing |
| 25 mm | About 10-100 ml |
| 30-33 mm | Larger volumes or viscous samples, higher flow |
Every EROHE syringe filter is sold in packs of 100 with the price per filter shown. Not sure which membrane fits your method? Request a free sample of the in-stock PTFE filter, or email sales@erohe-lab.com with your solvent and sample volume.
Questions buyers ask
Which membrane should I choose for cell culture media?
PES is widely used for media and buffers because it filters fast and binds little protein. PVDF also binds little protein; nylon suits aqueous and mixed solvent solutions.
Which vacuum filter size do I need?
Choose a unit at least as large as the volume you filter in one go: 150, 250, 500 or 1000 ml. Larger units have larger membranes (up to 91 mm) for faster flow.
0.22 µm or 0.45 µm for a vacuum filter?
0.22 µm removes bacteria and is used for sterile filtration of media and buffers. 0.45 µm is enough for clarifying solutions and degassing mobile phases.
What vacuum source can I use?
A laboratory vacuum pump or house vacuum line connected to the unit's vacuum port. Moderate vacuum avoids foaming of protein-containing media.