ER00853
PCR Plate Sealing Film, Clear, 100/pk
Clear
Lead time 3-4 weeks, ships from the Czech Republic (EU)
€113.00 per pack of 100 €1.13 per piece
PCR plate sealing film
Clear sealing film for PCR plates, used to close plate wells during thermal cycling and short-term storage.
It comes in packs of 100 sheets. Items not in stock have a lead time of 3 to 4 weeks.
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Choose the plate type for the job first: PCR or immunoassay. Then pick the format, well volume and colour.
Step 1: PCR plates: skirt and well volume
| Skirt | Choose it when |
|---|---|
| Non-skirted | Manual work on most standard thermal cycler blocks; the plate can be cut into sections |
| Semi-skirted | You want more rigidity and some robotic handling |
| Full-skirted | Robotic handling, plate sealers and automated readers |
0.1 ml (low-profile) wells suit low-profile and fast cycler blocks; 0.2 ml wells suit standard blocks. Check your cycler block before ordering. White wells reflect more light and are preferred for qPCR; clear wells let you see the liquid. Seal PCR plates with sealing film.
Step 2: ELISA plates: binding and colour
| Colour | Detection |
|---|---|
| Clear | Colorimetric (absorbance) assays |
| Black | Fluorescence; reduces background and crosstalk |
| White | Luminescence; reflects and maximises the signal |
High binding surfaces suit antibodies and proteins at low concentration; medium binding suits larger molecules and reduces non-specific binding. The plates are made of detachable strips (8 × 12 or 12 × 8), so you only use the wells you need.
Step 3: Reservoirs
Reagent reservoirs (10 to 100 ml, plain or divided) hold reagents for multichannel pipetting.
Questions buyers ask
How do I apply a PCR sealing film?
Peel off the backing, place the film over the plate and press firmly over every well and along the edges with a roller or paddle.
Why do seals sometimes lift during cycling?
Usually because the film was not pressed down over every well. Press again along the well rims before starting the run.
Can I cut the film for a part plate?
Yes, you can cut the film to cover only the wells you use.